Use of Fish Ventilation Frequency to Estimate Chronically Safe Toxicant Concentrations

Michael A. Cairns, Ronald R. Garton, Richard A. Tubb

Transactions of the American Fisheries Society · 1982 · 40 citations · 0 references

Concepts

Abstract

A 96-hour toxicity test with fish ventilation frequency as the response variable was developed to estimate safe zinc concentrations for steelhead Salmo gairdneri. Two Oregon steelhead strains were exposed to zinc at different water temperatures and total hardnesses (7, 12, and 17 C; 25 and 125 mg/liter as CaCO3). Ventilation frequencies enumerated from bioelectric potentials generated by buccal and opercular openings and closings showed significant increases at the highest test concentration in five of 10 tests. At 12 C and 25 mg/liter hardness, responses were detected at a zinc concentration of 144 μg/liter; the “safe” concentration determined in a chronic exposure of embryos and juveniles was between 444 and 819 μg zinc/liter. This indicates that ventilation-frequency tests are at least as sensitive as long-term toxicity tests and may be used to screen chemicals for potential harmful effects on fish.