Enzyme-Induced Chemiluminescence-Determination of Blood Glucose Using Luminol

Abstract

Abstract Hydrogen peroxide, produced by the interaction of glucose with immobilized glucose oxidase, reacts with the ferricyanide-luminol system to produce chemiluminescence linearly proportional to glucose concentration. The coupled reaction is used as a sensitive precise micro method for the determination of true blood glucose. The linear range of detection is 10−7 - 10−4 M glucose. The method correlates quantitatively with two glucose reference techniques.