Journal of Medical Entomology · 1992 · 123 citations · 0 references
Veterinary MedicineEntomologyImmunologyMedical EntomologyParasite ImmunologyParasitologyAntigen Detection AssaysHost-parasite Immune InteractionsMalariaParasitic Disease PathogenesisProtozoan Host-parasite InteractionsProtozoan ImmunologyDirect Enzyme-linked Immunosorbent AssayIndirect Enzyme-linked Immunosorbent AssayMalaria ImmunologyMalaria In Nonhuman PrimatesMalaria ParasitesPlasmodium FalciparumPlasmodium VivaxSporozoite Antigens
An enzyme-linked immunosorbent assay (ELISA) for the Plasmodium vivax-VK247 (variant) circumsporozoite (CS) protein was developed and evaluated using sporozoites produced by feeding mosquitoes on Thai patients with parasitologically confirmed P. vivax infections. The ELISA had a detection threshold of fewer than 50 sporozoites. Using this assay in conjunction with an ELISA for the VK210 polymorph, nearly 16% of the 235 P. vivax cases produced sporozoites positive only for the variant; 69% produced sporozoites positive only in the VK210 assay; and 15% were positive in both assays, indicating mixed infections. Twelve cases (5%) produced sporozoites negative in one assay and with unexpectedly low activity in the other ELISA, indicating the possibility of other CS protein polymorphs.