Articles: Efficacy of Iodophor Disinfection against Egg Surface-Associated Infectious Hematopoietic Necrosis Virus

Abstract

Abstract The efficacy of iodophor egg disinfection (IED) against egg surface-associated infectious hematopoietic necrosis virus (IHNV) on green and eyed eggs of rainbow trout (Oncorhynchus mykiss) was investigated. Viable IHNV was isolated from the surface of both green and eyed rainbow trout eggs that had been coated with 106 plaque-forming units (PFU) of IHNV per milliliter and disinfected in a static solution of iodophor containing 100 mg iodine/L for 10 and 60 min. Viable IHNV was also isolated from eyed rainbow trout eggs coated with 106 PFU IHNV/ mL and disinfected in circulated iodophor containing 100 mg iodine/L for 60 min. Although IHNV was recovered in each experiment, at least 99.98% of the virus was destroyed following IED.