Technical Notes: A Simple Procedure for Long-Term Storage and Preparation of Fish Cells for DNA Content Analysis Using Flow Cytometry

Abstract

Abstract A simple procedure for long-term storage and preparation offish cells for flow cytometric analysis of genome size or DNA content is described. The method has been used on a broad variety of cell or tissue types, including blood, soft organs, and muscle, and on fish ranging in size from 10 to 15 mm to over 500 mm in total length. No differences in estimated genome sizes or coefficients of variation have been observed with storage times of up to 8 months.