FEBS Letters · 1986 · 39 citations · 13 references
Life SciencesMolecular BiologyMolecular GeneticsGenomicsMolecular VirologyVirologyAnimal VirusesBacteriophage VectorsDna Virus BiologyViral GeneticsViral InfectionsCloningDna Fragment IsolationLambda Phage VectorsLong Terminal Repeat AnalysisLong Terminal Repeat RetrotransposonsRestriction EndonucleasesRetroviral VectorsRetrovirus Biology
Viral DNA of unintegrated closed circular form was isolated from a swine kidney cell line (SKL) which was infected with a porcine retrovirus Tsukuba-1 (PRetV) produced from a swine malignant lymphoma-derived cell line. Shimozuma-1 and cloned using a lambda phage vector, Charon 21A. One of ten independent clones contained the 8.3 kb DNA fragment as an insert, which was thought to be a full length of viral DNA molecule carrying a long terminal repeat (LTR) sequence. We have analyzed this insert by mapping the recognition sites of some restriction endonucleases by Southern blot hybridization with appropriate probes.
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