Growth characteristics of human skin fibroblastsin vitro.A simple experimental approach for the identification of hereditary adenomatosis of the colon and rectum

Abstract

Kinetic parameters of cell growth have been studied in cultured skin fibroblasts derived from individuals with hereditary adenomatosis of the colon and rectum. The growth parameters consisted of saturation density, plating density, cloning efficiency and colony-forming ability. The results suggest deployment of these selective assays to distinguish skin fibroblasts of presumptive ACR positive individuals from those of normal subjects in vitro.