Superheated Heavy Water as the Eluent for HPLC-NMR and HPLC-NMR-MS of Model Drugs

Abstract

Superheated heavy water can be used as the eluent for reversed-phase chromatography with on-line HPLC-NMR and HPLC-NMR-MS detection. The method has been demonstrated for the separation of model drugs (analgesics and caffeine) on a poly(styrene−divinylbenzene) stationary phase in isothermal and temperature-programmed modes. One- and two-dimensional spectra could be obtained with less interference from the mobile phase than with conventional HPLC-NMR eluents. Unlike supercritical fluid chromatography-NMR, the spectra could be measured at room temperature and atmospheric pressure. The combination of superheated water HPLC-NMR-MS chromatography enables both NMR and MS spectra to be obtained simultaneously for the same sample.