European Journal of Immunology · 1994 · 96 citations · 35 references
Life SciencesImmunologyPathologyCellular ImmunologyHypersensitivityInflammationCell-mediated ImmunityDelayed-type HypersensitivityHelper T Cell SubsetsT Cell ActivationT Cell BiologyT-cell Receptor GeneticsCytokine-mediated T Cell ActivationCytokine-mediated T Cell ResponsesInterferon-gamma ResponsesMacrophage Inflammatory ResponsesNaive T CellsT Cell Activation CytokinesT-cell Mediated ResponsesTransgenic Mice
We produced transgenic mice expressing T cell receptor-alpha beta chain genes, derived from the chicken ovalbumin (OVA)-specific I-Ad-restricted CD4+CD8- T helper cell clone 7-3-7. In transgenic mice with H-2d genetic background (Tg-d mice), delayed-type hypersensitivity (DTH) was induced in the hind footpad by one inoculation with OVA without any previous sensitization, suggesting that naive T cells have the potential to be involved in DTH response. Spleen cells from nonimmunized Tg-d mice showed a strong T cell proliferative response to in vitro stimulation with OVA. Furthermore, these spleen cells produce cytokines including interleukin(IL)-2, IL-3, interferon-gamma, granulocyte/macrophage colony-stimulating factor, macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta, which may play an important role in the attraction of mononuclear cells to an antigen-challenging site.
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Tolerance in T-cell-receptor transgenic mice involves deletion of nonmature CD4+8+ thymocytes
Paweł Kisielow, Horst Blüthmann, Uwe D. Staerz et al. · Nature · 1988 · 1.7K citations