Annals of the New York Academy of Sciences · 2008 · 67 citations · 12 references
MedicineMolecular BiologyOncologyEpigeneticsMolecular DiagnosticsMolecular OncologyCancer BiomarkersCancer EpigeneticsCancer Molecular DiagnosticsDna Methylation ProfilingEpigenetic Biomarker AnalysisTumor MarkersBiomarkers Of Gastric CancerCancer DiagnosticsCell-free Dna Methylation AnalysisCell-free Dna Methylation BiomarkersCirculating Cell-free Dna AnalysisCirculating Cell-free Dna MethylationPromoter MethylationPromoter Methylation Analysis
The concentration of cell-free DNA and promoter methylation status of the MGMT, p15, and hMLH1 genes were analyzed by a fluorescence-based assay and methylation-specific PCR (MSP) in the blood of gastric cancer patients (n= 20) and healthy subjects (n= 22). Gastric cancer patients were characterized by an increased concentration of circulating DNA in the plasma; the amount of cell-surface-bound DNA was not decreased compared with controls and amounted to 80 +/- 15% of the total circulating DNA. MSP analysis of three genes in the cell-surface-bound DNA permits the detection of gastric cancer patients with a sensitivity of 75% and a specificity of 54%. Thus, the cell-surface-bound DNA is a convenient source of DNA for MSP analysis of cancer-specific markers. The data on the presence of methylated DNA in plasma combined with the analysis of other cancer-related changes in DNA can significantly contribute to cancer diagnostics.
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Ahmedin Jemal, Freddie Bray, Jacques Ferlay et al. · CA A Cancer Journal for Clinicians · 2011 · 55K citations · Full text
Global Cancer Statistics, 2002
Donald Maxwell Parkin, Freddie Bray, Jacques Ferlay et al. · CA A Cancer Journal for Clinicians · 2005 · 18.4K citations · Full text