Journal of the American Chemical Society · 2009 · 627 citations · 24 references
Life SciencesBiotechnologyMolecular BiologyDrug DeliveryNanobiotechnologyNanoparticle Drug DeliveryNanomedicineNanoparticle DesignNucleic Acid DeliveryRna InterferenceSmall Interfering Rna BiologySmall Interfering Rna DeliveryConjugated OligonucleotidesGene Delivery NanoparticlesNanoparticle ConjugationOligonucleotide ConjugationRna Delivery NanoparticlesRna NanostructuresSmall Interfering Ribonucleic Acids (Sirnas)Transfection Reagents
We report the synthesis and characterization of polyvalent RNA-gold nanoparticle conjugates (RNA-Au NPs), nanoparticles that are densely functionalized with synthetic RNA oligonucleotides and designed to function in the RNAi pathway. The particles were rationally designed and synthesized to be free of degrading enzymes, have a high surface loading of siRNA duplexes, and contain an auxiliary passivating agent for increased stability in biological media. The resultant conjugates have a half-life six times longer than that of free dsRNA, readily enter cells without the use of transfection agents, and demonstrate a high gene knockdown capability in a cell model.
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