Competitive Binding Assay for Adenosine 3′,5′-Monophosphate Employing a Bovine Adrenal Protein: Application to Urine, Plasma and Tissues

Abstract

A rapid assay for adenosine 3′,5′-monophosphate has been described utilizing a crude bovine adrenal cortical preparation with high affinity and specificity. The working range of the assay is between 1–10 pmoles using an incubation volume of 1.5 ml. Resin, charcoal and membrane filter techniques to separate the bound and unbound fractions were all found to give similar results but the resin technique was preferred for its speed and simplicity. It was found that cAMP could be analyzed reproducibly in urine, plasma and tissues without extensive purification steps. Levels of the nucleotide in human fluids and animal tissues were found to be comparable to values reported by others using other techniques.