The determination of warfarin in animal tissues by electron-capture gas-liquid chromatography

Abstract

A method is described for the quantitative analysis of warfarin residues in animal tissues. An extract is made with acetone, purified by column chromatography and solvent partitioning and the final ether solution is methylated with diazomethane. The methylation products have been identified, and electron-capture gas-liquid chromatography has been used to determine 4-methylwarfarin, with decachlorobiphenyl as an internal standard. The recoveries have been determined by radiochemical techniques and shown to be suitable for the routine determination of warfarin at the 0.1 µg g–1 level in tissue.