Hemin/G-quadruplexes as DNAzymes for the fluorescent detection of DNA, aptamer–thrombin complexes, and probing the activity of glucose oxidase

Abstract

Hemin/G-quadruplex catalyzes the H(2)O(2)-mediated oxidation of Amplex Red to the fluorescent product resorufin. This process is implemented to develop hairpin nucleic acid structures for the detection of DNA, to probe the catalytic activity of glucose oxidase, to use the thrombin-aptamer complex as a catalytic readout structure, and to quantitatively analyze telomere chain composition.