The combinational use of <scp>CRISPR</scp>/Cas9‐based gene editing and targeted toxin technology enables efficient biallelic knockout of the α‐1,3‐galactosyltransferase gene in porcine embryonic fibroblasts

Abstract

These results demonstrated that the CRISPR/Cas9 system can efficiently induce the biallelic conversion of GGTA1 in the resulting somatic cells and is thus a promising tool for the creation of KO cloned piglets.