Journal of Biological Chemistry · 1999 · 119 citations · 11 references
Proteinlipid InteractionCpla2-beta CdnaMolecular RegulationCellular PhysiologyProtein ExpressionCpla2 FamilyCell SignalingProtein FunctionBiochemistryMembrane BiologyGene ExpressionCell BiologyProtein PhosphorylationPla2 ActivitiesSignal TransductionNatural SciencesCytosolic Phospholipase A2-βCellular BiochemistrySystems BiologyMedicineLipid Synthesis
We have isolated a cDNA encoding a 1012-amino acid polypeptide cPLA2-beta, that has significant homology with cPLA2-alpha in both the calcium-dependent lipid binding domain as well as in the catalytic domain. Transient expression of cPLA2-beta cDNA in COS cells results in an increase in calcium-dependent phospholipase A1 (PLA1) and PLA2 activities compared with vector-transfected cells. cPLA2-beta is markedly less selective for cleavage at sn-2 as compared with cPLA2-alpha and cPLA2-gamma. Northern analysis reveals a cPLA2-beta transcript of 8 kilobase pairs that is expressed in all the human tissues examined. With the identification of cPLA2-beta, the newly defined cPLA2 family now comprises three members that may have dramatically different mechanisms for regulation of expression and enzymatic activation.
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cPLA2 is phosphorylated and activated by MAP kinase
Lih‐Ling Lin, Markus Wartmann, Alice Lin et al. · Cell · 1993 · 1.9K citations
Reduced fertility and postischaemic brain injury in mice deficient in cytosolic phospholipase A2
Joseph V. Bonventre, Zhihong Huang, M. Reza Taheri et al. · Nature · 1997 · 841 citations
Medicine, Autophagy, Cell Death +12
Role of cytosolic phospholipase A2 in allergic response and parturition
Naonori Uozumi, Kazuhiko Kume, Takahide Nagase et al. · Nature · 1997 · 703 citations