Biotin Deficiency in the Rat

Abstract

Synthetic pyridoxine is almost inactive as a growth factor for S. Zizctis Rin a pyridoxine-free medium if heat sterilization is avoided. Autoclaving media which contain pyridoxine greatly increases its activity for this organism. The same effect was achieved in varying degrees by autoclaving pyridoxine at neutrality with individual amino acids. Cystine and glycine were most effective in producing this change.