Serological Detection of<i>Plasmodiophora brassicae</i>by Dot Immunobinding and Visualization of the Serological Reaction by Scanning Electron Microscopy

Abstract

The surface of the resting spores of P. brassicae, race 7, appeared smooth, while the dot immunobinding processed spores had a heavy, irregular coating, which was demonstrated to originate from incubation in the primary antiserum. Normal serum did not give rise to a coating on the resting spores. The antiserum of P. brassicae did not react with surface antigens of resting spores of Polymyxa