Properties of platelet concentrates prepared after extended whole blood holding time

Abstract

Extension of the maximum holding time for whole blood collected into a CPD-ADSOL system from 6 to 8 hours at ambient temperature under conditions that cause the temperature of the blood to decrease to 20 to 24 degrees C would facilitate the preparation of platelet concentrates (PCs). In this study, the properties of CPD-PCs prepared and stored for 5 days in PL-732 containers after various initial holding periods were assessed in two laboratories, designated Laboratory A and Laboratory B. Laboratory A found higher platelet-rich plasma (PRP) volumes (276 +/- 25 vs. 249 +/- 19 mL) and platelet yields (76 +/- 18 vs. 66 +/- 18 x 10(9) platelets) with 8-hour holds (n = 10) than with 1- to 2-hour holds (n = 10), although only the difference in PRP volumes was significant (p less than 0.05). No significant difference was observed in autologous in vivo recovery (54 +/- 11 vs. 47 +/- 9%) or survival (167 +/- 37 vs. 170 +/- 25 hrs), as calculated by gamma function using 111In as radiolabel. Laboratory B also found higher PRP volumes (304 +/- 31 vs. 279 +/- 37 mL) and platelet yields with 8-hour holds (n = 12) than with a 6-hour holds (n = 10) (88 +/- 26 vs. 77 +/- 27 x 10(9) platelets). No significant differences were found in morphology score, the extent of release of beta-thromboglobulin, the discharge of lactate dehydrogenase, or hypotonic shock response.(ABSTRACT TRUNCATED AT 250 WORDS)