Journal of the American Chemical Society · 2005 · 102 citations · 15 references
Natural SciencesBiochemistryMolecular BiologyCell BiophysicsMolecular BiophysicsMolecular ImagingFluorescence ImagingFluorescence SpectroscopyMolecular Probe DevelopmentPhotophysics Of Fluorescent ProbesFluorescence ProbesFluorescent Dye LabelingFluorescent Nucleic Acid ProbesFluorescent ProbesFluorescent Reporter SystemsMolecular Fluorescence ImagingParamagnetic SpeciesProtein Fluorescence
The compound NTA-DCF consists of two components, a dichlorofluorescein (DCF) reporter and a nitrilotriacetic acid (NTA) functionality. The latter binds polyhistidine sequences selectively through a bridging metal ion. The NTA-DCF conjugate has photophysical properties similar to those of the parent DCF fluorophore both by itself and as its nickel(II) complex. The insensitivity of the emission to paramagnetic ions allows the probe to label His6-tagged proteins fluorescently on the extracellular surfaces of HEK 293-T and HeLa cells.
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Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase
Irwin Chen, Mark Howarth, Weiying Lin et al. · Nature Methods · 2005 · 666 citations