Comparison of haematological parameters determined by the Sysmex KX - 2IN automated haematology analyzer and the manual counts

TLDR

The study aimed to assess the correlation between hematological parameters measured by the Sysmex KX‑21N automated analyzer and manual counting methods. Sixty subjects, including healthy individuals and patients with various blood disorders, had venous blood drawn into K3EDTA tubes and had hematological parameters measured by both the Sysmex KX‑21N automated analyzer and manual methods. The automated analyzer’s results correlated well with manual counts, showing significant differences in MCHC, hemoglobin, PCV, platelet, and WBC values yet maintaining positive correlations, while manual microscopy provided additional diagnostic detail.

Abstract

This study was designed to determine the correlation between heamatological parameters by Sysmex KX-21N automated hematology analyzer with the manual methods. Sixty (60) subjects were randomly selected from both apparently healthy subjects and those who have different blood disorders from the University of Teaching Hospital (UNTH), Ituku-Ozalla, Enugu, Enugu State, Nigeria. Three (3)mls of venous blood sample was collected aseptically from each subject into tri-potassium ethylenediamine tetra-acetic acid (K3EDTA) for the analysis of haematological parameters using the automated and the manual methods. The blood film report by the manual method showed that 50% of the subjects were normocytic-normochromic while the other 50% revealed different abnormal blood pictures. Also, there were statistically significant differences (p < 0.05) in mean cell hemoglobin concentrations (MCHC) between the two methods. Similarly, the mean (S.E) values of hemoglobin, packed cell volume, platelet and total white cell counts demonstrated statistically significant difference (p < 0.001) and correlated positively when both methods were compared. From the present study, it can be concluded that the automated hematology analyzer readings correlated well with readings by the standard manual method, although the latter method gave additional diagnostic information on the blood pictures. While patients' care and laboratory operations could be optimized by using manual microscopic examination as a reflective substitute for automated methods, usage of automated method would ease our workload and save time for patients.