Sporulation of <i>Gigaspora margarita</i> on root cultures of tomato

Abstract

Use of germinated spores of Gigaspora margarita as inoculum on tomato root culture plates routinely resulted in successful mycorrhizal infections in more than 85% of inoculated root cultures. Penetration into the root, vesicle formation, arbuscule production, and new spore formation were reproducibly observed. This represents the first description of spore formation by G. margarita under aseptic conditions.