Differentiation of barley yellow dwarf luteovirus serotypes infecting cereals and maize in Hungary

Abstract

— In 1994, a survey was carried out at 4 different locations in Hungary (Kiszombor, Kompolt, Martonvsr and Tplnszentkereszt) for the presence of barley yellow dwarf luteovirus (BYDV) serotypes in barley, wheat, triticale and maize. Leaf samples exhibiting symptoms of barley yellow dwarf virus were collected from cereal and maize plants. The number of samples collected was 119 from barley, 297 from wheat, 92 from triticale and 274 from maize. Diagnosis was done by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using RPV, RMV, MAV, PAV and SGV diagnostics (Agdia). We determined that, among the barley yellow dwarf luteovirus serotypes, the PAV serotype was dominant in cereals at all locations. In maize both the RPV and RMV serotypes were present at high rates.