¹H‐NMR Investigations on the Mutarotation of <i>N</i>‐Acetyl‐<scp>D</scp>‐neuraminic Acid

Abstract

Enzymatic liberation of N ‐acetyl‐α‐ D ‐neuraminic acid in an NMR spectrometer permitted the first determination of the mutarotation of this biochemically important sugar derivative which was formerly considered to be too fast to measure. Until recently it was assumed that only the β‐form is present in aqueous solution.