Interpretation of cardiac troponin T behaviour in size-exclusion chromatography

Abstract

cTnT has a Stokes radius of 33.7 A. Compared with globular calibrator proteins, intact cTnT elutes earlier than expected based solely on its molecular weight. For non-globular or uncharacterised proteins, Stokes radii should be used for correct interpretation of SEC data. By doing so, we were able to clearly demonstrate cTnT fragments.