European Journal of Endocrinology · 1962 · 35 citations · 0 references
MedicineBiochemistryEndocrinologyGeneral GynecologyMaternal-fetal MedicineReproductive MedicinePregnancyPregnancy PharmacologyChromogenic DetectionHydrolysisSilica Gel TreatmentUrinary Hormone MeasurementUrinary Reproductive BiomarkersUrinary Steroid Analysis
ABSTRACT A method suitable for the routine determination of urinary pregnanediol and pregnanetriol has been devised. It involves glucuronidase hydrolysis, extraction, and chromatography on disposable 1-g silica gel columns. The specificity of the pregnanediol determination is increased by acetylation and rechromatography on the same type of column. Sensitivity of the method is doubled by the use of sulfuric acid saturated with SO 2 gas as the chromogenic agent.