Infection and Stress Activation of Bean Chalcone Synthase Promoters in Transgenic Tobacco

Abstract

The object of this study was to define the inducibility of two CHS promoters in transgenic tobacco and to establish a system to investigate physical and biological agents that activate CHS promoter-driven transcription. ?enns between the 5'-flanking regulatory sequences of two CHS genes (CH58 and CHS15) and the GUS gene region encoding for β-glucoronidase were constructed and used to transform tobacco. One of these CHS promoters contains sequences that control expression associated with a bacterially induced hypersensitive response