An In Vitro Model of Posterior Capsular Opacity: SPARC and TGF-β2 Minimize Epithelial-to-Mesenchymal Transition in Lens Epithelium

Abstract

A simple IOL culture system was useful for the evaluation of the effects of SPARC and TGF-beta2 on PCO in vitro. The action of TGF-beta2 on LEC migration and proliferation is influenced by SPARC, a regulator of matrix-cell interactions. The results indicate a functional intersection between pathways activated by TGF-beta2 and SPARC in the formation of PCO.