Fluorescent Dyes as Probes of Voltage Transients in Neuron Membranes Progress Report

Abstract

Abstract Fluorescent dyes derived from anilino‐pyridinium are studied with respect to their application as probes of voltage‐transients in neuron membranes. Their photophysical properties are characterized in bulk solvents. The spectral features of their voltage‐sensitivity in membranes are determined. A mechanistic scheme of voltage‐sensitivity is proposed. The dyes are applied for optical recording in neurites of cultivated neurons of the leech. The relative sensitivity is evaluated from the fluorescence transients induced by hyperpolarization on the basis of the cable equation. The pervasion of a neuritic tree by action potentials is observed.