Thermostable Glycosynthases and Thioglycoligases Derived from <i>Thermotoga maritima</i> β‐Glucuronidase

Abstract

By using active-site mutants of the Thermotoga maritima β-glucuronidase, glucuronic acid-containing glycosides and thioglycosides have been made in high yields. These enzymes have potential use in the chemoenzymatic synthesis of a range of glycoconjugates, such as glycosaminoglycans (X=OH, NAc, H), that contain β-linked glucuronic and galacturonic acids. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2006/z600028_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.