Kinase‐Actuated Immunoaggregation of Peptide‐Conjugated Gold Nanoparticles

Abstract

A simple and sensitive one-step approach for the rapid colorimetric detection of v-Src kinase using functionalized gold nanoparticles (NPs) in solution is reported. The generic concept operates without labeled ATP-derivatives, and signals are recorded directly by spectrophotometry. For the first time, surface plasmon resonance spectroscopy is applied to an NP system and compared to a semi-quantitative NP reaction model that was developed to determine the association rate constants and the rate-limiting step of the NP agglutination process. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.