Uptake of Tritium-Labeled Norepinephrine in Brain and Other Tissues of Cat in vitro

Abstract

Slices of cat cerebral cortex, heart, and spleen that have been incubated in media containing approximately 5 to 25 mmicrog of dl-norepinephrine-7H(8) per milliliter contain levels of isotopic amine greater than those in the medium. The effects of norepinephrine concentration, reserpine, and ouabain on the uptake suggest that the amine enters cells both by diffusion and by a concentrating miechanism that is saturated at low levels of norepinephrine. The drugs inhibit the latter.