Human lymphoblastoid cell line producing protective monoclonal IgG₁, χ anti‐tetanus toxin

Abstract

Peripheral blood lymphocytes from an individual, recently boosted with tetanus toxoid (TT), were transformed with Epstein-Barr virus. No antigen-specific selection nor stimulation of B cells was performed prior to transformation. One stable cell line, designated CLB-Hu-TT-1, was established. This cell line has a doubling time of 24 h and yields 10 micrograms/ml of a monoclonal IgG1, kappa anti-TT antibody in bulk cultures. The antibody is biologically active in that it can protect mice against the effects of tetanus toxin. The cell line has been characterized with regard to some cytoplasmic and membrane markers.