Metal‐Free Bacterial Haloperoxidases as Unusual Hydrolases: Activation of H₂O₂ by the Formation of Peracetic Acid

Abstract

How does the catalytic set-up of a hydrolase activate H2O2 for enzymatic oxidations? The oxidizing species is a peracid, as these studies with the metal-free haloperoxidases CPO-P and CPO-T clearly show. The analogy between the hydrolase and haloperoxidase reactions is elucidated in the reactions below.