Detection of α‐amylase isoenzymes by means of two‐dimensional electrophoresis followed by blue starch staining

Abstract

Abstract α‐Amylase isoenzymes present in human body fluids were separated by two‐dimensional electrophoresis in the absence of denaturing agents and detected by blue starch staining. Human body fluid samples (5 sera, 2 salivas, 1 pancreatic juice and 1 urine) were subjected to the analysis. Two to six activity spots of α‐amylase were detected for each sample. The spots were classified into four main species by their isoelectric points (p I ) 6.9, 6.5, 5.8 and 5.3. All the α‐amylase isoenzymes showed apparent molecular weights above 140 000.