A Bod Sensor Using<i>Klebsiella Oxytoca</i>AS1

Abstract

Abstract A BOD sensor using Klebsiella oxytoca AS 1 was investigated with special emphasis on the effect of cell properties, such as the number, and growth phase, of immobilized cells, on the sensor response. The sensor response was almost independent of the cell number in the case of low BOD solutions, whereas the response increased with increasing cell number when high BOD solutions were used. The growth phase of the cells immobilized in the membrane affected the sensor response, especially when the membrane had been stored at 30 °C for long time. Cells immobilized at the beginning of the stationary growth phase were the most effective in terms of both the sensitivity and preservability. 1 he response of K. oxytoca sensor to various substrates, including sugars and amino acids, was similar to that of a Trichosporon cutaneum sensor. The K. oxytoca sensor showed a higher resistance to some toxic substances, such as phenol, compared to T. cutaneum sensor.