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Two-color immunofluorescence using a fluorescence-activated cell sorter.
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1977
Year
Immunocytochemical TechniqueEngineeringImmunologySingle WavelengthImmunophenotypingFlow CellBiomedical EngineeringImmunochemistryClinical ChemistryMicrofluidicsBiofluid DynamicImmunoglobulin DMolecular ImagingBiophysicsFluorescence ImagingBiomedical AnalysisBiophotonicsImmunoglobulin MCell BiologyFluorescence-activated Cell SorterMedicine
A technique for the analysis of fluorescein and rhodamine in a flow system using a single wavelength of excitation is described. Both optics and electronics are used to discriminate the fluorescein and rhodamine signals. This technique has been used to study the relationship between immunoglobulin M and immunoglobulin D on mouse splenic lymphocytes.