Characterization of flavonoids in Millettia nitida var. hirsutissima by HPLC/DAD/ESI-MS

Abstract

<i>Millettia nitida</i> var. <i>hirsutissima</i> is a Chinese herbal medicine used for the treatment of gynecological diseases. An HPLC/DAD/ESI-MS ^<i>n</i> method was established for the rapid separation and characterization of bioactive flavonoids in <i>M. nitida</i> var. <i>hirsutissima</i>. A total of 32 flavonoids were detected, of which 14 compounds were unambiguously characterized by comparing their retention time, UV, and MS spectra with those of the reference standards, and the others were tentatively identified based on their tandem mass spectrometry fragmentation data obtained in the negative ionization mode on line. Nineteen of these compounds characterized were reported from this plant for the first time.