Non-destructive methods of detecting Curtobacterium flaccumfaciens pv. flaccumfaciens in mungbean seeds

Abstract

An indirect immunofluorescent assay (IF) with specific monoclonal antibodies (MAbs) and a semi‐selective agar medium for Curtobacterium flaccumfaciens pv. flaccumfaciens, developed in this study, were compared with foliar symptoms and microscopic bacterial ooze for detection of this pathogen in mungbean seed 6 d and 28 d after germination. The IF method detected more infected seedlings than the other three methods at both samplings. Symptomless carriers of C. flaccumfaciens pv. flaccumfaciens in mungbean were detectable only by the IF method and, less frequently, by plating out on media. Poor agreement between the IF and other methods was found. The IF method gave the best agreement in the detection of the pathogen between early and late samplings of individual germinated seed. Currently, the IF technique with a specific MAb is being used for selecting clean seedlings for production of disease‐free seed.