Perforin, Granzyme B, and Fas Ligand for Molecular Diagnosis of Acute Renal-Allograft Rejection: Analyses on Serial Biopsies Suggest Methodological Issues

Abstract

Two lytic pathways are activated in biopsies when AR occurs shortly after Tx, whereas the P/GB mechanism prevails if it occurs later on. Only P and FL in biopsies might be able to predict AR diagnosis, but with a considerable variability in each sample, possibly due to the small portion of tissue core, which may be inadequate for molecular diagnosis. CTL expression in PBL does not correlate with histological AR.