Electrophoresis · 2002 · 28 citations · 18 references
PharmacyPharmaceutical AnalysisPharmaceuticsAnalytical ChemistryChromatographic AnalysisMass Spectrometric AnalysisAnalytical ChromatographyChromatographic Analysis Of PharmaceuticalsChromatographyDegradation Product AnalysisInstrumental AnalysisMass SpectrometryBioanalytical ChromatographyChromatographic Drug AnalysisChromatographic Separation Of DegradantsLiquid Chromatography-mass SpectrometryLiquid Chromatography-mass Spectrometry AnalysisLiquid Chromatography-mass Spectrometry Method DevelopmentMicellar Electrokinetic ChromatographySteroid Degradation Products
Studies have been performed to evaluate whether an on-line partial filling-micellar electrokinetic chromatography (PF-MEKC) system could be applied to a recently developed MEKC method for the separation of ibuprofen, codeine and one of the degradation products. Attempts to couple the PF-MEKC system to MS have also been performed. SDS concentration, micellar zone length and concentration of acetonitrile in the buffer were optimized using factorial design. When a small micelle zone was injected directly after the sample introduction, the results improved markedly. The MS parameters have not been optimized, but the studies show promising results for the use of PF-MEKC-mass spectrometry for identification of the degradation products.
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