HPLC Method for Screening of Steroidal Saponins and Rutin as Biologically Active Compounds in<i>Tribulus Terrestris</i>L.

Abstract

An improvement method for extraction of steroidal saponins and rutin of Tribulus terrestris L. (Zygophyllaceae) was evaluated. It is shown that the ultrasound extraction is faster, easier, solvent-saving, more reliable and effective method than the conventional heat reflux extraction method.Under optimized conditions, the main biologically active compounds protodioscin, prototribestin, dioscin and rutin were extracted from Tribulus terrestris with different origin and analyzed by revesed phase HPLC with UV detector.