Indole-3-acetic Acid Levels of Plant Tissue as Determined by a New High Performance Liquid Chromatographic Method

Abstract

A method for the analysis of indole-3-acetic acid (IAA) in plant extracts has been developed based on high performance liquid chromatography separation of IAA on a microparticulate strong anion exchange column followed by quantitation with two selective detectors: an electrochemical, carbon paste amperometric detector and/or a fluorescence detector. The detection limit for IAA is less than 1 nanogram with the fluorescence detector and less than 50 picograms with the electrochemical detector.The IAA levels are reported for various tissues of wheat, pinto beans, soybeans, cotton, and corn.