CYTOGENETIC DEMONSTRATION OF PERMANENT TOLERANCE IN ADULT OUTBRED DOGS

Abstract

Two male dogs were given supralethal whole-body irradiation followed by an infusion of bone marrow obtained from unrelated female donors. Three and 4 years later, chromosomal analyses of cells obtained from bone marrow, peripheral blood, and lymph nodes from these males showed in all analyzable cells the presence of the typical female karyotype. It is concluded that in these clinically well, adult animals a true chimeric state and mutual tolerance between graft and host has been achieved on a long-term basis.