Enantioselective separations of chiral molecules by μ‐HPLC and SFC on microbore and packed microcapillary columns

Abstract

Abstract New results obtained with microbore or microcapillary columns packed with DACH‐DNB CSP (spherical silica, 5 μm) using conventional LC eluents or supercritical fluids are reported. Examples of direct chromatographic separations of complex mixtures of stereo‐isomers on: a) long, packed microbore or microcapillary columns and b) several columns coupled in series are shown; in the latter case, a home‐made calculation procedure (Simulation for Multi‐Column Chromatography–SMCC) was used to quickly select the optimal conditions (kind of stationary phases and relative columns length).