Effects of Thermal-Induced Respiratory Alkalosis on Blood Ionized Calcium Levels in the Domestic Hen

Abstract

An experiment was conducted to determine the change with time in the blood ionized calcium concentration of hens exposed to an acute heat stress. Five hens were surgically fitted with carotid artery cannulae and placed in cages inside a temperature-controlled chamber. Blood samples were drawn before (23 C), during (35 C), and after (23 C), a 3-hr heat-stress exposure. Whole blood ionized calcium, blood gas, plasma pyruvate, and lactate were determined. Respiratory alkalosis developed 1 hr after the start of heat exposure (35 C). Approximately 1 hr later, there was a concomitant decline in blood pH as plasma lactate and pyruvate concentration increased (P less than .05). In addition, the blood ionized calcium level was reduced (P less than .05) by 19% and was negatively correlated (P less than .05) with plasma pyruvate (-.77) and lactate (-.81). These results suggest that changes in acid-base balance during heat stress reduce the blood ionized calcium level, which may in turn limit the availability of calcium for egg shell formation.