FEBS Letters · 1996 · 51 citations · 21 references
Life SciencesBiochemistryMolecular BiologyMitochondrial BiologyMitochondrial GeneticsProtein BiochemistryHeat Stress ToleranceMitochondrial BiochemistryMitochondrial BioenergeticsMitochondrial FunctionMitochondrial PhysiologyMitochondrial Stress ResponsesHeat Shock Cognate 70Heat Shock ResponseHeat Stress MitigationHsp70 Chaperone SystemLuciferase ReportersProtein Aggregation AnalysisYeast Mitochondrial BiologyYeast Mitochondrial Genetics
The role of the mitochondrial Hsp70 system in the prevention of heat-induced protein aggregation was studied in isolated mitochondria from Saccharomyces cerevisiae. Firefly luciferase was employed as a thermolabile tester protein. After shift to 40 degrees Celsius transient increase of mt-Hsp70/luciferase complex was observed, which required functional Mdj1p and Mge1p, the mitochondrial homologues of DnaJ and GrpE. The kinetics of luciferase aggregation, however, were not influenced by mutations in either mt-Hsp70 or Mge1p. Only the absence of Mdj1p led to enhanced protein aggregation. Thus, a central role in the transient protection against heat stress is attributed to this mitochondrial DnaJ homologue.
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Guide to Yeast Genetics and Molecular Biology
C. P. Kurtzman, Christine Guthrie, Gerald R. Fink · Mycologia · 1993 · 2.4K citations
Protein disaggregation mediated by heat-shock protein Hspl04
Dawn A. Parsell, Anthony S. Kowal, Mike A. Singer et al. · Nature · 1994 · 870 citations