Effective qPCR methodology to quantify the expression of virulence genes in <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i>

Abstract

The methods used in this study gave more reliable expression data using qPCR than previously published methods. The quantification and expression dynamics of virulence genes will contribute to a better understanding of how Aer. salmonicida interacts with its host and the environment, and therefore to the prevention of epizootics due to this pathogen.