Immunological studies on mouse mammary tumors. IV. Extraction and solubilization of transplantation antigen of mouse mammary tumor

Abstract

Abstract Antigens present in mouse mammary tumor MM2 reacting with tumor‐specific antibodies were extracted with 0.2% deoxycholate. After the removal of deoxycholate by dialysis, most antigens were precipitated by centrifugation at 105,000 × g for 60 min. Hyperimmunization of C3H/He mice with these antigens induced heightened resistance against MM2. Antibodies appeared in the serum after a booster injection of deoxycholate extract of tumor cells was given. Antigens were solubilized from deoxycholate extract with distilled water. Purified soluble antigen inhibited the neutralization activity of the tumor‐specific antibody to MM2 tumor. From these observations, it is concluded that transplantation antigens of isografted mammary tumors in C3H/He mice are present in deoxycholate extracts of MM2 tumor.