Hormonal Control of Orthophosphate Incorporation into Phospholipids of Barley Aleurone Layers

Abstract

Gibberellic acid added to isolated barley aleurone layers enhances orthophosphate incorporation into chloroform-methanol-soluble compounds. The effect is measurable at 4 to 6 hours after the addition of gibberellic acid and reaches a maximum after 8 to 12 hours. The increase in the rate of orthophosphate incorporation is 3- to 5-fold over the rate in control layers incubated without gibberellic acid.The gibberellic acid enhancement of the rate of phospholipid labeling is inhibited within 1 to 2 hours by cycloheximide, 6-methylpurine, and abscisic acid.The increase in labeling of phospholipids occurs throughout the subcellular fractions rather than being restricted to a specific fraction or organelle. The increase in radioactivity in phospholipids as shown by thin layer chromatography is due to a proportional increase in all phospholipids.The enhancement by gibberellic acid of the rate of phospholipid labeling may be required for the subsequent production of gibberellic acid-induced hydrolases.